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MEK1/2 (phospho Ser218/222) antibody

Anti-MEK1/2 (phospho Ser218/222) antibody used in Western Blot (WB). GTX48615
Anti-MEK1/2 (phospho Ser218/222) antibody used in Western Blot (WB). GTX48615

Cat No. GTX48615

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Mouse, Rat, Zebrafish, Bovine, Dog, Chicken, Monkey, Xenopus
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

100 μl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser218,222 of human MEK 1/2. Note: The phosphosites in human MEK 1/2 are Ser218 and Ser222; the phosphosites in mouse MEK 1/2 are Ser217 and Ser221

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Mitogen-Activated Protein Kinase Kinase 1 , Cfc3 , Mapkk1 , Mek1 , Mkk1 , Prkmk1 , Map2K1

Background

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.

Database

Research Area

DATA IMAGES

Anti-MEK1/2 (phospho Ser218/222) antibody used in Western Blot (WB). GTX48615

GTX48615 WB Image

Western Blot: MEK1/2 Phosphospecific Antibody [GTX48615] - Western Blot of NIH 3T3 cell lysates showing specific immunolabeling of the ~45kDa MEK 1/2 protein phosphorylated at Ser218 and Ser222. The cells were either serum starved (Control) or incubated in the presence of serum (Serum). Immunolabeling of an additional band at ~95kDa was also observed.

Anti-MEK1/2 (phospho Ser218/222) antibody used in Western Blot (WB). GTX48615

GTX48615 WB Image

REFERENCE

REVIEW

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SDS
Glycerol.pdf