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MEK1/2 (phospho Ser221) antibody

Anti-MEK1/2 (phospho Ser221) antibody used in Western Blot (WB). GTX52337

Cat No. GTX52337

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC

Reactivity

Human, Mouse, Rat
Package
100 μg ($319)

APPLICATION

Application Note

WB 1:500-1:2000, IHC 1:50-1:200

Calculated MW

43 kDa. ( Note )

Specificity/Sensitivity

MAP2K1

PROPERTIES

Form

Liquid

Buffer

phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human MEK1/2 around the phosphorylation site of Ser221

Purification

Antigen affinity purification

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Mitogen-Activated Protein Kinase Kinase 1 , Cfc3 , Mapkk1 , Mek1 , Mkk1 , Prkmk1 , Map2K1

Background

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MEK1/2 (phospho Ser221) antibody used in Western Blot (WB). GTX52337

GTX52337 WB Image

Western blot analysis of MEK1/2 phosphorylation expression in UV treated Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf