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MEK1 + MEK2 antibody [GT1721]

Anti-MEK1 + MEK2 antibody [GT1721] used in Western Blot (WB). GTX630542
Anti-MEK1 + MEK2 antibody [GT1721] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630542
Anti-MEK1 + MEK2 antibody [GT1721] used in Western Blot (WB). GTX630542
Anti-MEK1 + MEK2 antibody [GT1721] used in Western Blot (WB). GTX630542
Anti-MEK1 + MEK2 antibody [GT1721] used in Western Blot (WB). GTX630542
Anti-MEK1 + MEK2 antibody [GT1721] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630542

Cat. No. GTX630542

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1721

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Human, Drosophila, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all MEK1 + MEK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MEK1 + MEK2. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888215

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-MEK1 + MEK2 antibody [GT1721] used in Western Blot (WB). GTX630542

GTX630542 WB Image

MEK2 antibody [GT1721] detects MEK2 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract | D. 30 μg HepG2 whole cell lysate/extract
10 % SDS-PAGE
MEK2 antibody [GT1721] (GTX630542) dilution: 1:5000

Anti-MEK1 + MEK2 antibody [GT1721] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630542

GTX630542 ICC/IF Image

MEK1 + MEK2 antibody [GT1721] detects MEK1 + MEK2 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MEK1 + MEK2 stained by MEK1 + MEK2 antibody [GT1721] (GTX630542) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-MEK1 + MEK2 antibody [GT1721] used in Western Blot (WB). GTX630542

GTX630542 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with MEK1 + MEK2 antibody [GT1721] (GTX630542) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-MEK1 + MEK2 antibody [GT1721] used in Western Blot (WB). GTX630542

GTX630542 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with MEK1 + MEK2 antibody [GT1721] (GTX630542) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-MEK1 + MEK2 antibody [GT1721] used in Western Blot (WB). GTX630542

GTX630542 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with MEK1 + MEK2 antibody [GT1721] (GTX630542) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-MEK1 + MEK2 antibody [GT1721] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630542

GTX630542 ICC/IF Image

MEK1 + MEK2 antibody [GT1721] detects MEK1 + MEK2 protein at cytoplasm by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MEK2 stained by MEK1 + MEK2 antibody [GT1721] (GTX630542) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations