APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
NIH-3T3 , JC
Predict Reactivity
Zebrafish, Rabbit, Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human MEK1 + MEK2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10724117
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
MAPKK1 antibody , MEK1 antibody , MKK1 antibody , PRKMK1 antibody , MAP2K1 antibody , "protein kinase , mitogen-activated , kinase 1 (MAP kinase kinase 1) antibody" , ERK activator kinase 1 antibody , MEK 1 antibody , MAP kinase kinase 1 antibody , MAPK/ERK kinase 1 antibody , dual specificity mitogen-activated protein kinase kinase 1 antibody , MAPKK 1 antibody , mitogen-activated protein kinase kinase 1 antibody , FLJ26075 antibody , MAPKK2 antibody , MEK2 antibody , MKK2 antibody , PRKMK2 antibody , MAP2K2 antibody , MAP kinase kinase 2 antibody , ERK activator kinase 2 antibody , "mitogen-activated protein kinase kinase 2 , p45 antibody" , MAPK/ERK kinase 2 antibody , dual specificity mitogen-activated protein kinase kinase 2 antibody , mitogen-activated protein kinase kinase 2 antibody
Research Area
DATA IMAGES
|
GTX121942 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with MEK1 + MEK2 antibody (GTX121942) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
|
|
|
GTX121942 ICC/IF Image
MEK1 + MEK2 antibody detects MEK1 + MEK2 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: MEK1 + MEK2 stained by MEK1 + MEK2 antibody (GTX121942) diluted at 1:500.Blue: Hoechst 33342 staining.
|
|
|
GTX121942 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MEK1 + MEK2 antibody (GTX121942) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX121942 WB Image
Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
10% SDS PAGE
GTX121942 diluted at 1:2000
|
|
|
GTX121942 ICC/IF Image
MEK1+2 antibody detects MEK1+2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MEK1+2 protein stained by MEK1+2 antibody (GTX121942) diluted at 1:500.
Blue: Hoechst 33342 staining.
|
|
|
GTX121942 IHC-P Image
MEK1+2 antibody detects MEK1+2 protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
MEK1+2 antibody (GTX121942) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
REFERENCE
REVIEW
There are currently no reviews for MEK1 + MEK2 antibody (GTX121942). Be the first to share your experience with this product.
