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MEK1 + MEK2 antibody

Anti-MEK1 + MEK2 antibody used in Western Blot (WB). GTX121942
Anti-MEK1 + MEK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121942
Anti-MEK1 + MEK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX121942
Anti-MEK1 + MEK2 antibody used in Western Blot (WB). GTX121942
Anti-MEK1 + MEK2 antibody used in Western Blot (WB). GTX121942
Anti-MEK1 + MEK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121942

Cat. No. GTX121942

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat, Dog
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

NIH-3T3 , JC

Predict Reactivity

Zebrafish, Rabbit, Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MEK1 + MEK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10724117

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MAPKK1 antibody , MEK1 antibody , MKK1 antibody , PRKMK1 antibody , MAP2K1 antibody , "protein kinase , mitogen-activated , kinase 1 (MAP kinase kinase 1) antibody" , ERK activator kinase 1 antibody , MEK 1 antibody , MAP kinase kinase 1 antibody , MAPK/ERK kinase 1 antibody , dual specificity mitogen-activated protein kinase kinase 1 antibody , MAPKK 1 antibody , mitogen-activated protein kinase kinase 1 antibody , FLJ26075 antibody , MAPKK2 antibody , MEK2 antibody , MKK2 antibody , PRKMK2 antibody , MAP2K2 antibody , MAP kinase kinase 2 antibody , ERK activator kinase 2 antibody , "mitogen-activated protein kinase kinase 2 , p45 antibody" , MAPK/ERK kinase 2 antibody , dual specificity mitogen-activated protein kinase kinase 2 antibody , mitogen-activated protein kinase kinase 2 antibody

Research Area

DATA IMAGES

Anti-MEK1 + MEK2 antibody used in Western Blot (WB). GTX121942

GTX121942 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
10% SDS PAGE
GTX121942 diluted at 1:2000

Anti-MEK1 + MEK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121942

GTX121942 ICC/IF Image

MEK1+2 antibody detects MEK1+2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MEK1+2 protein stained by MEK1+2 antibody (GTX121942) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-MEK1 + MEK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX121942

GTX121942 IHC-P Image

MEK1+2 antibody detects MEK1+2 protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
MEK1+2 antibody (GTX121942) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MEK1 + MEK2 antibody used in Western Blot (WB). GTX121942

GTX121942 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MEK1 + MEK2 antibody (GTX121942) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MEK1 + MEK2 antibody used in Western Blot (WB). GTX121942

GTX121942 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with MEK1 + MEK2 antibody (GTX121942) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-MEK1 + MEK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121942

GTX121942 ICC/IF Image

MEK1 + MEK2 antibody detects MEK1 + MEK2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MEK1 + MEK2 stained by MEK1 + MEK2 antibody (GTX121942) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169