ICC/IF analysis of mouse embryonic fibroblasts (MEFs) using GTX24750 MEK1 + MEK2 (phospho Ser222) antibody. The cell is actively dividing. Blue represents chromosomes in anaphase of mitotic cell division. Green shows mitotic spindle expressing phosphorylated MEK1, 2.

WB (peptide competition) analysis of NIH3T3 cells treated with 50 ng/mL PDGF for 15 minutes (Lane 2-5) using GTX24750 MEK1 + MEK2 (phospho Ser222) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphoserine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

WB analysis of whole cell extracts (30 ug lysate) of A549 (Lane 1), treated with EGF (200 ng/ml for 10 min) (Lane 2), and treated with Afatinib (0.5 uM for 16 h) followed by EGF (200 ng/ml for 10 min) using GTX24750 MEK1 + MEK2 (phospho Ser222) antibody.
Dilution : 1:1000