GeneTex
United States (US)

MEK1 antibody

Comparison
GTX102391_WB_19011506_803.jpg
GTX102391_40100_20160323_IHC-P_R_19011506_844.jpg
GTX102391_40100_WB_M_brain_19011506_375.jpg
GTX102391_40100_IFA_19011506_215.jpg
GTX102391_40100_20161117_WB_competitor_watermark_19011506_358.jpg

Cat No. GTX102391

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse brain

Predict Reactivity

Zebrafish, Rabbit, Bovine, Dog, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MEK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mitogen-Activated Protein Kinase Kinase 1,Cfc3,Mapkk1,Mek1,Mkk1,Prkmk1,Map2K1

Background

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102391_WB_19011506_803.jpg

GTX102391 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
GTX102391 diluted at 1:1000

GTX102391_40100_20160323_IHC-P_R_19011506_844.jpg

GTX102391 IHC-P Image

MEK1 antibody detects MEK1 protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
MEK1 antibody (GTX102391) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102391_40100_WB_M_brain_19011506_375.jpg

GTX102391 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX102391 diluted at 1:3000

GTX102391_40100_IFA_19011506_215.jpg

GTX102391 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using MEK1(GTX102391) antibody (Green) at 1:200 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

GTX102391_40100_20161117_WB_competitor_watermark_19011506_358.jpg

GTX102391 WB Image

Comparison

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membranes were blotted with MEK1 antibody (GTX102391) diluted at 1:1000 and competitor's antibody (SC-219) diluted by 1:200.
*The competitor is not affiliated with GeneTex and does not endorse this product.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169