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MEK1 antibody

KO/KD Comparison Overexpression
Anti-MEK1 antibody used in Western Blot (WB). GTX134234
Anti-MEK1 antibody used in Western Blot (WB). GTX134234
Anti-MEK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX134234
Anti-MEK1 antibody used in Western Blot (WB). GTX134234
Anti-MEK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134234
Anti-MEK1 antibody used in Western Blot (WB). GTX134234
Anti-MEK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX134234

Cat. No. GTX134234

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all MEK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS , MEK1-transfected 293T

Predict Reactivity

Rabbit, Bovine, Dog, Chicken, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of rat MEK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887240

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen activated protein kinase kinase 1 , Mek1

Cellular Localization

Cytoplasm,Nucleus,Membrane

Background

kinase that activates Mapk3 (Erk1) and Mapk1 (Erk2) kinases [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-MEK1 antibody used in Western Blot (WB). GTX134234

GTX134234 WB Image

KO/KD

Wild-type (WT) and MEK1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MEK1 antibody (GTX134234) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-MEK1 antibody used in Western Blot (WB). GTX134234

GTX134234 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MEK1 antibody (GTX134234) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MEK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX134234

GTX134234 IHC-P Image

MEK1 antibody detects MEK1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
MEK1 stained by MEK1 antibody (GTX134234) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MEK1 antibody used in Western Blot (WB). GTX134234

GTX134234 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with MEK1 antibody (GTX134234) diluted at 1:500 and competitor's antibody (sc-219) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-MEK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134234

GTX134234 ICC/IF Image

MEK1 antibody detects MEK1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MEK1 stained by MEK1 antibody (GTX134234) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-MEK1 antibody used in Western Blot (WB). GTX134234

GTX134234 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MEK1 antibody (GTX134234) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MEK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX134234

GTX134234 IHC-P Image

MEK1 antibody detects MEK1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
MEK1 stained by MEK1 antibody (GTX134234) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

SH Chen et al. Neurotherapeutics 2024; 21 (3) : e00336 Ultrahigh frequency transcutaneous electrical nerve stimulation for neuropathic pain alleviation and neuromodulation
Application : IHC-Fr
Su C.H. et al. Journal of Functional Foods 2021; 86 Betulin suppresses TNF-α and IL-1β production in osteoarthritis synovial fibroblasts by inhibiting the MEK/ERK/NF-κB pathway.
Application : WB
Reactivity : Human
Lee H.P. et al. ournal of Functional Foods 2021; 86 : 104712 Cordycerebroside A suppresses VCAM-dependent monocyte adhesion in osteoarthritis synovial fibroblasts by inhibiting MEK/ERK/AP-1 signaling.
Application : WB
Reactivity : Human
Krzysiek-Maczka G et al. Microorganisms 2020; 8 (10) Long-Term Helicobacter pylori Infection Switches Gastric Epithelium Reprogramming Towards Cancer Stem Cell-Related Differentiation Program in Hp-Activated Gastric Fibroblast-TGFβ Dependent Manner.
Application : WB
Reactivity : Rat
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169