APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000 - 1:3000 |
| 1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAP2K1 (NP_002746.1).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
mitogen-activated protein kinase kinase 1 , CFC3 , MAPKK1 , MEK1 , MKK1 , PRKMK1
Cellular Localization
Cytoplasm,Nucleus,Membrane
Background
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX35212 WB Image
WB analysis of various sample lysates using GTX35212 MEK1 antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:3000
Loading : 25μg per lane
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GTX35212 IHC-P Image
IHC-P analysis of rat brain tissue using GTX35212 MEK1 antibody.
Dilution : 1:100
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GTX35212 IHC-P Image
IHC-P analysis of mouse kidney tissue using GTX35212 MEK1 antibody.
Dilution : 1:100
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GTX35212 WB Image
WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX35212 MEK1 antibody.
Dilution : 1:1000
Loading : 25μg per lane
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REFERENCE
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REVIEW
There are currently no reviews for MEK1 antibody (GTX35212). Be the first to share your experience with this product.
