APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:1000 |
| 1:100-1:200 |
| 1:50-1:100 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Mouse, Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide sequence around phosphorylation site of serine 221 (A-N-S(p)-F-V) derived from human MEK1.
Purification
Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
mitogen-activated protein kinase kinase 1 , CFC3 , MAPKK1 , MEK1 , MKK1 , PRKMK1
Cellular Localization
Cytoplasm,Nucleus,Membrane
Background
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX50216 WB Image
WB analysis of extracts from HeLa cells treated with calf intestinal phosphatase (CIP) using GTX50216 MEK1 (phospho Ser221) antibody.
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GTX50216 ICC/IF Image
ICC/IF analysis of methanol-fixed HeLa cells using GTX50216 MEK1 (phospho Ser221) antibody.
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GTX50216 IHC-P Image
IHC-P analysis of human breast carcinoma tissue using GTX50216 MEK1 (phospho Ser221) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide
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GTX50216 WB Image
WB analysis of extracts from HeLa cells untreated or treated with EGF using GTX50216 MEK1 (phospho Ser221) antibody.
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REFERENCE
There are currently no references for MEK1 (phospho Ser221) antibody (GTX50216). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MEK1 (phospho Ser221) antibody (GTX50216). Be the first to share your experience with this product.
