APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:1000 |
| 1:50 - 1:100 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Mouse, Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human MEK1 around the phosphorylation site of serine 221 (A-N-Sp-F-V).
Purification
Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum
Conjugation
Unconjugated
RRID
AB_11164335
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CFC3,MAP2K1,MAPKK1,MEK1,MKK1,PRKMK1,mitogenactivated protein kinase kinase 1,mitogen-activated protein kinase kinase 1
Cellular Localization
Cytoplasm,Cytoskeleton,Microtubule organizing center,Centrosome,Spindle pole body,Nucleus,Membrane
Background
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX79016 WB Image
WB analysis of UV treated Jurkat cell lysate using GTX79016 MEK1 (phospho Ser221) antibody (Lane 3 and 4) and MEK1 antibody (Lane 1 and 2).
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GTX79016 IHC-P Image
IHC-P analysis of human breast carcinoma tissue using GTX79016 MEK1 (phospho Ser221) antibody.
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REFERENCE
There are currently no references for MEK1 (phospho Ser221) antibody (GTX79016). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MEK1 (phospho Ser221) antibody (GTX79016). Be the first to share your experience with this product.
