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MEK1 (phospho Ser298) antibody [GT1305]

Recombinant antibody Orthogonal
Anti-MEK1 (phospho Ser298) antibody [GT1305] used in Western Blot (WB). GTX03217
Anti-MEK1 (phospho Ser298) antibody [GT1305] used in Western Blot (WB). GTX03217
Anti-MEK1 (phospho Ser298) antibody [GT1305] used in Western Blot (WB). GTX03217

Cat. No. GTX03217

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1305

Isotype

 IgG

Application

 WB

Reactivity

 Human, Rat
Package
100 μl ($419)
See all MEK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.08 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding S298 of human MEK1.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase kinase 1 , CFC3 , MAPKK1 , MEK1 , MKK1 , PRKMK1

Cellular Localization

Cytoplasm , Nucleus , Membrane

Background

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MEK1 (phospho Ser298) antibody [GT1305] used in Western Blot (WB). GTX03217

GTX03217 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MEK1 (phospho Ser298) antibody [GT1305] (GTX03217) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-MEK1 (phospho Ser298) antibody [GT1305] used in Western Blot (WB). GTX03217

GTX03217 WB Image

Orthogonal

WB analysis of HeLa cells were treated by CIP(20uL/400ul) at 37ºC for 1 hour or treated by PMA (200 nM) at 37ºC for 30 minutes after serum-starvation overnight using GTX03217 MEK1 (phospho Ser298) antibody [GT1305].
Dilution : 1:1000
Loading : 25μg per lane

Anti-MEK1 (phospho Ser298) antibody [GT1305] used in Western Blot (WB). GTX03217

GTX03217 WB Image

Orthogonal

WB analysis of C6 cells were treated by CIP(20uL/400ul) at 37ºC for 1 hour or treated by PMA (200 nM) at 37ºC for 30 minutes after serum-starvation overnight using GTX03217 MEK1 (phospho Ser298) antibody [GT1305].
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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Package List Price ($)
$ 419