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MEK1 (phospho Ser298) antibody

Orthogonal
Anti-MEK1 (phospho Ser298) antibody used in IHC (Paraffin sections) (IHC-P). GTX25613
Anti-MEK1 (phospho Ser298) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25613
Anti-MEK1 (phospho Ser298) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25613
Anti-MEK1 (phospho Ser298) antibody used in IHC (Paraffin sections) (IHC-P). GTX25613
Anti-MEK1 (phospho Ser298) antibody used in Western Blot (WB). GTX25613

Cat. No. GTX25613

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat
Package
50 μl ($399)
See all MEK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:250
IHC-P 1:20-1:200
FACS 1:20
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human MEK1 that contains serine 298. The sequence is conserved in many species including mouse, rat, chimp, hamster, and rabbit.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_380596

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase kinase 1 , CFC3 , MAPKK1 , MEK1 , MKK1 , PRKMK1

Cellular Localization

Cytoplasm,Nucleus,Membrane

Background

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MEK1 (phospho Ser298) antibody used in IHC (Paraffin sections) (IHC-P). GTX25613

GTX25613 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX25613 MEK1 (phospho Ser298) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

Anti-MEK1 (phospho Ser298) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25613

GTX25613 ICC/IF Image

ICC/IF analysis of A549 cells (plated on fibronectin , serum starved overnight then stimulated with serum for 1 hour) using GTX25613 MEK1 (phospho Ser298) antibody.
Green : Primary antibody
Red : Actin
Blue : cell nucleus

Anti-MEK1 (phospho Ser298) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25613

GTX25613 ICC/IF Image

ICC/IF analysis of A549 cells using GTX25613 MEK1 (phospho Ser298) antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes
Dilution : 1:250 dilution in 1% BSA incubated for 3 hours at room temperature

Anti-MEK1 (phospho Ser298) antibody used in IHC (Paraffin sections) (IHC-P). GTX25613

GTX25613 IHC-P Image

IHC-P analysis of human kidney tissue using GTX25613 MEK1 (phospho Ser298) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

Anti-MEK1 (phospho Ser298) antibody used in Western Blot (WB). GTX25613

GTX25613 WB Image

Orthogonal

WB (peptide competition) analysis of NIH3T3 cells treated with 50 ng/mL PDGF for 15 minutes (Lane 2-7) using GTX25613 MEK1 (phospho Ser298) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphoserine-containing peptide (Lane 4) or the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 6) eliminates the signal further verifying that the antibody is phospho-specific.

REFERENCE

Application Reference

Hsieh WY et al. PLoS One 2019; 14 (1) : e0210656 Renal chymase-dependent pathway for angiotensin II formation mediated acute kidney injury in a mouse model of aristolochic acid I-induced acute nephropathy.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399