WB (peptide competition) analysis of NIH3T3 cells treated with 50 ng/mL PDGF for 15 minutes (Lane 2-7) using GTX25613 MEK1 (phospho Ser298) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphoserine-containing peptide (Lane 4) or the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 6) eliminates the signal further verifying that the antibody is phospho-specific.

IHC-P analysis of human kidney tissue using GTX25613 MEK1 (phospho Ser298) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

ICC/IF analysis of A549 cells using GTX25613 MEK1 (phospho Ser298) antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes
Dilution : 1:250 dilution in 1% BSA incubated for 3 hours at room temperature

ICC/IF analysis of A549 cells (plated on fibronectin , serum starved overnight then stimulated with serum for 1 hour) using GTX25613 MEK1 (phospho Ser298) antibody.
Green : Primary antibody
Red : Actin
Blue : cell nucleus

IHC-P analysis of human colon carcinoma tissue using GTX25613 MEK1 (phospho Ser298) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100