ICC/IF analysis of A549 cells using GTX25612 MEK1 (phospho Thr292) antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes
Dilution : 1:250 dilution in 1% BSA incubated for 3 hours at room temperature

IHC-P analysis of human kidney tissue using GTX25612 MEK1 (phospho Thr292) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

WB (peptide competition) analysis of NIH3T3 cells treated with 50 ng/mL PDGF for 15 minutes (Lanes 2-5) using GTX25612 MEK1 (phospho Thr292) antibody with a generic phosphothreonine-containing peptide (Lane 3), the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 4), or the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.