WB analysis of EGF-treated A431 (A), EGF-treated HeLa (B) whole cell lysates using GTX32249 MEK1 (phospho Thr292) antibody.

ICC/IF analysis of formalin-fixed HeLa cells using GTX32249 MEK1 (phospho Thr292) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32249 MEK1 (phospho Thr292) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)