MEK1 (phospho Thr292) antibody

Anti-MEK1 (phospho Thr292) antibody used in IHC (Paraffin sections) (IHC-P). GTX32249

Cat No. GTX32249

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human

See all MEK1 products

Datasheet

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500 - 1:1000
ICC/IF	1:100 - 1:500
IHC-P	1:100 - 1:200

Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of MEK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Mitogen-Activated Protein Kinase Kinase 1,Cfc3,Mapkk1,Mek1,Mkk1,Prkmk1,Map2K1

Cellular Localization

Centrosome,Cytoplasm,Cytoskeleton,Membrane,Microtubule organizing center,Nucleus,Spindle pole body

Background

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]

Database

Gene ID: 5604
UniProt: Q02750

Research Area

DATA IMAGES

GTX32249 WB Image

WB analysis of EGF-treated A431 (A), EGF-treated HeLa (B) whole cell lysates using GTX32249 MEK1 (phospho Thr292) antibody.

GTX32249 ICC/IF Image

ICC/IF analysis of formalin-fixed HeLa cells using GTX32249 MEK1 (phospho Thr292) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

GTX32249 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32249 MEK1 (phospho Thr292) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)