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MEK2 antibody [N1C1]

Anti-MEK2 antibody [N1C1] used in Western Blot (WB). GTX102198
Anti-MEK2 antibody [N1C1] used in Western Blot (WB). GTX102198
Anti-MEK2 antibody [N1C1] used in Western Blot (WB). GTX102198
Anti-MEK2 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX102198

Cat. No. GTX102198

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Dog
Package
100 μl ($399),
25 μl ($169)
See all MEK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

Molt-4 , NIH-3T3

Predict Reactivity

Rat, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MEK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950859

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mitogen-activated protein kinase kinase 2 , CFC4 , MAPKK2 , MEK2 , MKK2 , PRKMK2

Background

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MEK2 antibody [N1C1] used in Western Blot (WB). GTX102198

GTX102198 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with MEK2 antibody (GTX102198) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-MEK2 antibody [N1C1] used in Western Blot (WB). GTX102198

GTX102198 WB Image

Sample(30 ug whole cell lysate)
A:MOLT4 (GTX27912)
10% SDS PAGE
GTX102198 diluted at 1:1000

Anti-MEK2 antibody [N1C1] used in Western Blot (WB). GTX102198

GTX102198 WB Image

Sample (30 ug of whole cell lysate)

A:NIH-3T3
10% SDS PAGE

GTX102198 diluted at 1:1000

Anti-MEK2 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX102198

GTX102198 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using MAP2K2(GTX102198) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169