Immunohistochemical analysis of paraffin-embedded human breast cancer, using MAP2K2(GTX102198) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)

A:NIH-3T3
10% SDS PAGE

GTX102198 diluted at 1:1000

Sample(30 ug whole cell lysate)
A:MOLT4 (GTX27912)
10% SDS PAGE
GTX102198 diluted at 1:1000

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with MEK2 antibody (GTX102198) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.