GeneTex
United States (US)

MEK2 antibody

KO/KD Overexpression
GTX102186_40289_20180316_WB_KO_watermark_19011506_821.jpg
GTX102186_WB_19011506_824.jpg
GTX102186_40289_IHC_19011506_202.jpg
GTX102186_40289_20180914_WB_B_19011506_424.jpg
GTX102186_40289_IFA_19011506_437.jpg

Cat No. GTX102186

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:25000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji

Predict Reactivity

Mouse, Rat, Bovine, Dog, Chicken, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.97mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MEK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ26075 antibody, MAPKK2 antibody, MEK2 antibody, MKK2 antibody, PRKMK2 antibody, MAP2K2 antibody, MAP kinase kinase 2 antibody, ERK activator kinase 2 antibody, "mitogen-activated protein kinase kinase 2, p45 antibody", MAPK/ERK kinase 2 antibody, dual specificity mitogen-activated protein kinase kinase 2 antibody, mitogen-activated protein kinase kinase 2 antibody

Background

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102186_40289_20180316_WB_KO_watermark_19011506_821.jpg

GTX102186 WB Image

KO/KD

Wild-type (WT) and MEK2 knockout (KO) 293T cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with MEK2 antibody (GTX102186) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX102186_WB_19011506_824.jpg

GTX102186 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
GTX102186 diluted at 1:1000

GTX102186_40289_IHC_19011506_202.jpg

GTX102186 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using MEK2(GTX102186) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102186_40289_20180914_WB_B_19011506_424.jpg

GTX102186 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with MEK2 antibody (GTX102186) diluted at 1:25000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX102186_40289_IFA_19011506_437.jpg

GTX102186 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using MEK2(GTX102186) antibody at 1:500 dilution.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169