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MEK2 antibody

Anti-MEK2 antibody used in Western Blot (WB). GTX102186
Anti-MEK2 antibody used in Western Blot (WB). GTX102186
Anti-MEK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX102186
Anti-MEK2 antibody used in Western Blot (WB). GTX102186
Anti-MEK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102186

Cat No. GTX102186

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:25000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji

Predict Reactivity

Mouse, Rat, Bovine, Dog, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.97 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MEK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mitogen-activated protein kinase kinase 2 , CFC4 , MAPKK2 , MEK2 , MKK2 , PRKMK2

Background

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MEK2 antibody used in Western Blot (WB). GTX102186

GTX102186 WB Image

Wild-type (WT) and MEK2 knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MEK2 antibody (GTX102186) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MEK2 antibody used in Western Blot (WB). GTX102186

GTX102186 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
GTX102186 diluted at 1:1000

Anti-MEK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX102186

GTX102186 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using MEK2(GTX102186) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MEK2 antibody used in Western Blot (WB). GTX102186

GTX102186 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MEK2 antibody (GTX102186) diluted at 1:25000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-MEK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102186

GTX102186 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using MEK2(GTX102186) antibody at 1:500 dilution.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf