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MEK2 (phospho Thr394) antibody

Anti-MEK2 (phospho Thr394) antibody used in Western Blot (WB). GTX50096
Anti-MEK2 (phospho Thr394) antibody used in IHC (Paraffin sections) (IHC-P). GTX50096
Anti-MEK2 (phospho Thr394) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50096

Cat No. GTX50096

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of threonine 394 (P-G-T(p)-P-T) derived from human MEK2.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mitogen-activated protein kinase kinase 2 , CFC4 , MAPKK2 , MEK2 , MKK2 , PRKMK2

Cellular Localization

Cytoplasm,Membrane

Background

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, cognitive disability, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MEK2 (phospho Thr394) antibody used in Western Blot (WB). GTX50096

GTX50096 WB Image

WB analysis of extracts from HepG2 and HeLa cells untreated or treated with UV using GTX50096 MEK2 (phospho Thr394) antibody.

Anti-MEK2 (phospho Thr394) antibody used in IHC (Paraffin sections) (IHC-P). GTX50096

GTX50096 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50096 MEK2 (phospho Thr394) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-MEK2 (phospho Thr394) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50096

GTX50096 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50096 MEK2 (phospho Thr394) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319