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MEK3 antibody [N1C3]

Anti-MEK3 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107529
Anti-MEK3 antibody [N1C3] used in Western Blot (WB). GTX107529
Anti-MEK3 antibody [N1C3] used in Western Blot (WB). GTX107529
Anti-MEK3 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107529
Anti-MEK3 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107529
Anti-MEK3 antibody [N1C3] used in Western Blot (WB). GTX107529
Anti-MEK3 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107529

Cat. No. GTX107529

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all MEK3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

Mouse liver

Predict Reactivity

Rat, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.66 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MEK3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950861

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase kinase 3 , MAPKK3 , MEK3 , MKK3 , PRKMK3 , SAPKK-2 , SAPKK2

Background

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MEK3 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107529

GTX107529 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using MEK3(GTX107529) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MEK3 antibody [N1C3] used in Western Blot (WB). GTX107529

GTX107529 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
10% SDS PAGE
GTX107529 diluted at 1:500

Anti-MEK3 antibody [N1C3] used in Western Blot (WB). GTX107529

GTX107529 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MEK3 antibody [N1C3] (GTX107529) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MEK3 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107529

GTX107529 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using MEK3(GTX107529) antibody at 1:200 dilution.

Anti-MEK3 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107529

GTX107529 IHC-P Image

MEK3 antibody [N1C3] detects MEK3 protein at cytoplasm in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
MEK3 antibody [N1C3] (GTX107529) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MEK3 antibody [N1C3] used in Western Blot (WB). GTX107529

GTX107529 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MEK3 antibody [N1C3] (GTX107529) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MEK3 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107529

GTX107529 IHC-P Image

MEK3 antibody [N1C3] detects MEK3 protein at cytoplasm in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
MEK3 antibody [N1C3] (GTX107529) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169