APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , H1299 , HeLa , HepG2 , Raji , mouse liver
Predict Reactivity
Rat, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris-Glycine (pH7), 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.66 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human MEK3. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1950861
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
mitogen-activated protein kinase kinase 3 , MAPKK3 , MEK3 , MKK3 , PRKMK3 , SAPKK-2 , SAPKK2
Background
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-MEK3 antibody [N1C3] used in Western Blot (WB). GTX107529](/templates/default/js/lightbox/images/loading.gif "Anti-MEK3 antibody [N1C3] used in Western Blot (WB). GTX107529")
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GTX107529 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MEK3 antibody [N1C3] (GTX107529) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX107529 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MEK3 antibody [N1C3] (GTX107529) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX107529 WB Image
Sample (50 ug of whole cell lysate)
A: mouse liver
10% SDS PAGE
GTX107529 diluted at 1:500
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GTX107529 IHC-P Image
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using MEK3(GTX107529) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX107529 IHC-P Image
MEK3 antibody [N1C3] detects MEK3 protein at cytoplasm in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
MEK3 antibody [N1C3] (GTX107529) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX107529 ICC/IF Image
Immunofluorescence analysis of methanol-fixed HeLa, using MEK3(GTX107529) antibody at 1:200 dilution.
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GTX107529 IHC-P Image
MEK3 antibody [N1C3] detects MEK3 protein at cytoplasm in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
MEK3 antibody [N1C3] (GTX107529) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
There are currently no references for MEK3 antibody [N1C3] (GTX107529). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MEK3 antibody [N1C3] (GTX107529). Be the first to share your experience with this product.
![Anti-MEK3 antibody [N1C3] used in Western Blot (WB). GTX107529](/upload/website/prouct_img/small/GTX107529/GTX107529_40037_20220311_WB_22031423_609.jpg "Anti-MEK3 antibody [N1C3] used in Western Blot (WB). GTX107529")
![Anti-MEK3 antibody [N1C3] used in Western Blot (WB). GTX107529](/upload/website/prouct_img/small/GTX107529/GTX107529_40037_20220311_WB_2_22031423_757.jpg "Anti-MEK3 antibody [N1C3] used in Western Blot (WB). GTX107529")
![Anti-MEK3 antibody [N1C3] used in Western Blot (WB). GTX107529](/upload/website/prouct_img/small/GTX107529/GTX107529_40037_WB_M_liver_19011506_892.jpg "Anti-MEK3 antibody [N1C3] used in Western Blot (WB). GTX107529")
![Anti-MEK3 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107529](/upload/website/prouct_img/small/GTX107529/GTX107529_40037_IHC_19011506_746.jpg "Anti-MEK3 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107529")
![Anti-MEK3 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107529](/upload/website/prouct_img/small/GTX107529/GTX107529_40037_20150420_IHC_M_2_19011506_699.jpg "Anti-MEK3 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107529")
![Anti-MEK3 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107529](/upload/website/prouct_img/small/GTX107529/GTX107529_IFA_19011506_907.jpg "Anti-MEK3 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107529")
![Anti-MEK3 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107529](/upload/website/prouct_img/small/GTX107529/GTX107529_40037_20150420_IHC_M_19011506_394.jpg "Anti-MEK3 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107529")
![Anti-MEK3 antibody [N1C3] used in Western Blot (WB). GTX107529](/upload/website/prouct_img/normal/GTX107529/GTX107529_40037_20220311_WB_22031423_609.jpg "Anti-MEK3 antibody [N1C3] used in Western Blot (WB). GTX107529")
![Anti-MEK3 antibody [N1C3] used in Western Blot (WB). GTX107529](/upload/website/prouct_img/normal/GTX107529/GTX107529_40037_20220311_WB_2_22031423_757.jpg "Anti-MEK3 antibody [N1C3] used in Western Blot (WB). GTX107529")
![Anti-MEK3 antibody [N1C3] used in Western Blot (WB). GTX107529](/upload/website/prouct_img/normal/GTX107529/GTX107529_40037_WB_M_liver_19011506_892.jpg "Anti-MEK3 antibody [N1C3] used in Western Blot (WB). GTX107529")
![Anti-MEK3 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107529](/upload/website/prouct_img/normal/GTX107529/GTX107529_40037_IHC_19011506_746.jpg "Anti-MEK3 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107529")
![Anti-MEK3 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107529](/upload/website/prouct_img/normal/GTX107529/GTX107529_40037_20150420_IHC_M_2_19011506_699.jpg "Anti-MEK3 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107529")
![Anti-MEK3 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107529](/upload/website/prouct_img/normal/GTX107529/GTX107529_IFA_19011506_907.jpg "Anti-MEK3 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107529")
![Anti-MEK3 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107529](/upload/website/prouct_img/normal/GTX107529/GTX107529_40037_20150420_IHC_M_19011506_394.jpg "Anti-MEK3 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107529")