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MEK3 (phospho Ser189) antibody

Anti-MEK3 (phospho Ser189) antibody used in Western Blot (WB). GTX50203
Anti-MEK3 (phospho Ser189) antibody used in Western Blot (WB). GTX50203
Anti-MEK3 (phospho Ser189) antibody used in IHC (Paraffin sections) (IHC-P). GTX50203
Anti-MEK3 (phospho Ser189) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50203

Cat No. GTX50203

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 189 (V-D-S(p)-V-A) derived from human MEK3.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mitogen-activated protein kinase kinase 3 , MAPKK3 , MEK3 , MKK3 , PRKMK3 , SAPKK-2 , SAPKK2

Background

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MEK3 (phospho Ser189) antibody used in Western Blot (WB). GTX50203

GTX50203 WB Image

WB analysis of extracts from 3T3 cells treated with Anisomycin or calf intestinal phosphatase (CIP) using GTX50203 MEK3 (phospho Ser189) antibody .

Anti-MEK3 (phospho Ser189) antibody used in Western Blot (WB). GTX50203

GTX50203 WB Image

WB analysis of extracts from HeLa cells untreated or treated with PMA using GTX50203 MEK3 (phospho Ser189) antibody .

Anti-MEK3 (phospho Ser189) antibody used in IHC (Paraffin sections) (IHC-P). GTX50203

GTX50203 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50203 MEK3 (phospho Ser189) antibody .
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-MEK3 (phospho Ser189) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50203

GTX50203 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50203 MEK3 (phospho Ser189) antibody .

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf