MEK3 (phospho Ser189) antibody

Cat. No. GTX79010

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human
Package	100 μg ($399)

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Datasheet

View product citations for antibody GTX79010 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500 - 1:1000
IHC-P	1:50 - 1:100

Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human MKK3 around the phosphorylation site of serine 189 (V-D-Sp-V-A).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11172068

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MAP2K3,MAPKK3,MEK3,MKK3,PRKMK3,SAPKK2,mitogenactivated protein kinase kinase 3,mitogen-activated protein kinase kinase 3

Background

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008]

Database

Gene ID: 5606 MAP2K3
UniProt: P46734 MAP2K3

Research Area

DATA IMAGES

GTX79010 WB Image

WB analysis of MDA-MB-435 cells untreated or treated with UV lysates using GTX79010 MEK3 (phospho Ser189) antibody (Lane 3 and 4) and MEK3 antibody (Lane 1 and 2).

GTX79010 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX79010 MEK3 (phospho Ser189) antibody.

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 399

Qty