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MEK3 (phospho Ser189) antibody

Anti-MEK3 (phospho Ser189) antibody used in IHC (Paraffin sections) (IHC-P). GTX86304
Anti-MEK3 (phospho Ser189) antibody used in Western Blot (WB). GTX86304

Cat No. GTX86304

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μg ($359)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500~1:1000
IHC-P 1:50~1:100
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human MEK3 (173-222).

Purification

Purified by antigen-affinity chromatography. Non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

MAP2K3 , MAPKK3 , MEK3 , MKK3 , PRKMK3 , SAPKK2 , mitogenactivated protein kinase kinase 3 , mitogen-activated protein kinase kinase 3

Background

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MEK3 (phospho Ser189) antibody used in IHC (Paraffin sections) (IHC-P). GTX86304

GTX86304 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX86304 MEK3 (phospho Ser189) antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MEK3 (phospho Ser189) antibody used in Western Blot (WB). GTX86304

GTX86304 WB Image

WB analysis of MDA-MB-435 cell lysates using GTX86304 MEK3 (phospho Ser189) antibody. The lane on the right is blocked with the synthesized peptide.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 359