APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1/500 - 1/2000 |
1/200 - 1/1000 |
1/200 - 1/400 |
1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.03% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Purified recombinant fragment of human MEK6 expressed in E. Coli.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
mitogen-activated protein kinase kinase 6 , MAPKK6 , MEK6 , MKK6 , PRKMK6 , SAPKK-3 , SAPKK3
Cellular Localization
Nucleus,Cytoplasm,Cytoplasm, cytoskeleton
Background
This gene encodes a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX60396 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX60396 MEK6 antibody [3H12C8]. Green : MEK6 Blue: DRAQ5 fluorescent DNA dye
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GTX60396 FACS Image
FACS analysis of HeLa cells using GTX60396 MEK6 antibody [3H12C8]. Green : MEK6 Purple : negative control
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GTX60396 ELISA Image
ELISA analysis of antigen using GTX60396 MEK6 antibody [3H12C8].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng
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GTX60396 WB Image
WB analysis of HepG2 (1), MCF-7 (2) and NIH3T3 (3) cell lysate using GTX60396 MEK6 antibody [3H12C8].
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REFERENCE
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REVIEW
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