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MEK6 antibody [N1C3]

Anti-MEK6 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107445
Anti-MEK6 antibody [N1C3] used in Western Blot (WB). GTX107445
Anti-MEK6 antibody [N1C3] used in Western Blot (WB). GTX107445
Anti-MEK6 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107445
Anti-MEK6 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107445

Cat. No. GTX107445

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all MEK6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Predict Reactivity

Rat, Zebrafish, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.31 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MEK6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950862

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase kinase 6 , MAPKK6 , MEK6 , MKK6 , PRKMK6 , SAPKK-3 , SAPKK3

Background

This gene encodes a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MEK6 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107445

GTX107445 IHC-P Image

MEK6 antibody [N1C3] detects MEK6 protein at cytoplasm and nucleus in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
MEK6 antibody [N1C3] (GTX107445) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MEK6 antibody [N1C3] used in Western Blot (WB). GTX107445

GTX107445 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with MEK6 antibody [N1C3] (GTX107445) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MEK6 antibody [N1C3] used in Western Blot (WB). GTX107445

GTX107445 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MEK6 antibody [N1C3] (GTX107445) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MEK6 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107445

GTX107445 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using MEK6(GTX107445) antibody at 1:200 dilution.

Anti-MEK6 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107445

GTX107445 IHC-P Image

Immunohistochemical analysis of paraffin-embedded N87 xenograft, using MEK6(GTX107445) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Li L et al. Pathol Oncol Res 2018; (Epub) CAP2 is a Valuable Biomarker for Diagnosis and Prognostic in Patients with Gastric Cancer.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169