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MEK6 antibody [N1C3]

Anti-MEK6 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107445
Anti-MEK6 antibody [N1C3] used in Western Blot (WB). GTX107445
Anti-MEK6 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107445
Anti-MEK6 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107445

Cat No. GTX107445

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

293T , HeLa , HepG2 , Molt-4

Predict Reactivity

Rat, Zebrafish, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.31 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MEK6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mitogen-Activated Protein Kinase Kinase 6,Mapkk6,Mek6,Mkk6,Prkmk6,Sapkk-3,Sapkk3,Map2K6

Background

This gene encodes a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-MEK6 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107445

GTX107445 IHC-P Image

MEK6 antibody [N1C3] detects MEK6 protein at cytoplasm and nucleus in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
MEK6 antibody [N1C3] (GTX107445) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MEK6 antibody [N1C3] used in Western Blot (WB). GTX107445

GTX107445 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
GTX107445 diluted at 1:1000

Anti-MEK6 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107445

GTX107445 IHC-P Image

Immunohistochemical analysis of paraffin-embedded N87 xenograft, using MEK6(GTX107445) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MEK6 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107445

GTX107445 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using MEK6(GTX107445) antibody at 1:200 dilution.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169