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MEK7 antibody [N1C3]

Anti-MEK7 antibody [N1C3] used in Western Blot (WB). GTX103563
Anti-MEK7 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103563

Cat No. GTX103563

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Rat, Zebrafish, Sheep, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MEK7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

mitogen-activated protein kinase kinase 7 , JNKK2 , MAPKK7 , MEK , MEK 7 , MKK7 , PRKMK7 , SAPKK-4 , SAPKK4

Cellular Localization

Nucleus , Cytoplasm

Background

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found, but only one transcript variant has been supported and defined. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-MEK7 antibody [N1C3] used in Western Blot (WB). GTX103563

GTX103563 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
10% SDS PAGE
GTX103563 diluted at 1:1000

Anti-MEK7 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103563

GTX103563 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human adenocarcinoma, using MEK7(GTX103563) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169