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MEKK2 antibody [4B4]

Anti-MEKK2 antibody [4B4] used in Western Blot (WB). GTX60504
Anti-MEKK2 antibody [4B4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60504
Anti-MEKK2 antibody [4B4] used in ELISA (ELISA). GTX60504

Cat. No. GTX60504

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4B4

Isotype

 IgG1

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($409)
See all MEKK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human MAP3K2 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase kinase kinase 2 , MEKK2 , MEKK2B

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a member of serine/threonine protein kinase family. This kinase preferentially activates other kinases involved in the MAP kinase signaling pathway. This kinase has been shown to directly phosphorylate and activate Ikappa B kinases, and thus plays a role in NF-kappa B signaling pathway. This kinase has also been found to bind and activate protein kinase C-related kinase 2, which suggests its involvement in a regulated signaling process. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MEKK2 antibody [4B4] used in Western Blot (WB). GTX60504

GTX60504 WB Image

WB analysis of HEK293 (1) and MEKK2 (AA: 148-359)--hIgGFc transfected HEK293 (2) cell lysate using GTX60504 MEKK2 antibody [4B4].

Anti-MEKK2 antibody [4B4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60504

GTX60504 ICC/IF Image

ICC/IF analysis of 3T3-L1 cells using GTX60504 MEKK2 antibody [4B4].
Green : MEKK2
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-MEKK2 antibody [4B4] used in ELISA (ELISA). GTX60504

GTX60504 ELISA Image

ELISA analysis of antigen using GTX60504 MEKK2 antibody [4B4].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 409