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MEKK3 antibody [N3C2], Internal

Anti-MEKK3 antibody [N3C2], Internal used in Western Blot (WB). GTX111498
Anti-MEKK3 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111498

Cat No. GTX111498

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

3xFlag-human MAP3K3-transfected 293T

Predict Reactivity

Mouse, Rat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MEKK3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11178437

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mitogen-activated protein kinase kinase kinase 3 , MAPKKK3 , MEKK3

Background

This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MEKK3 antibody [N3C2], Internal used in Western Blot (WB). GTX111498

GTX111498 WB Image

MEKK3 antibody [N3C2], Internal detects MEKK3 protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg whole cell extract of 3xFlag-human MAP3K3-transfected 293T cells
7.5 % SDS-PAGE
MEKK3 antibody [N3C2], Internal (GTX111498) dilution: 1:5000

Anti-MEKK3 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111498

GTX111498 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using MEKK3(GTX111498) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

There are currently no references for MEKK3 antibody [N3C2], Internal (GTX111498). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for MEKK3 antibody [N3C2], Internal (GTX111498). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169