GeneTex
United States (US)

MEKK3 antibody [N3C3]

GTX107945_40016_IHC1_19011507_706.jpg
GTX107945_40016_IHC2_19011507_248.jpg
GTX107945_40016_20150417_IHC_M_2_19011507_150.jpg
GTX107945_40016_20150417_IHC_M_19011507_897.jpg
GTX107945_40016_WB_B_19011507_973.jpg

Cat No. GTX107945

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

3xFlag-human MAP3K3-transfected 293T

Predict Reactivity

Rat, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.71mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MEKK3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MAPKKK3 antibody, MEKK3 antibody, MAP3K3 antibody, MAP/ERK kinase kinase 3 antibody, MEKK 3 antibody, mitogen-activated protein kinase kinase kinase 3 antibody, MEK kinase 3 antibody, MAPK/ERK kinase kinase 3 antibody

Background

This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX107945_40016_IHC1_19011507_706.jpg

GTX107945 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using MEKK3(GTX107945) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX107945_40016_IHC2_19011507_248.jpg

GTX107945 IHC-P Image

Immunohistochemical analysis of paraffin-embedded S-G xenograft, using MEKK3(GTX107945) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX107945_40016_20150417_IHC_M_2_19011507_150.jpg

GTX107945 IHC-P Image

MEKK3 antibody [N3C3] detects MEKK3 protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
MEKK3 antibody [N3C3] (GTX107945) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX107945_40016_20150417_IHC_M_19011507_897.jpg

GTX107945 IHC-P Image

MEKK3 antibody [N3C3] detects MEKK3 protein at cytoplasm and nucleus in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
MEKK3 antibody [N3C3] (GTX107945) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX107945_40016_WB_B_19011507_973.jpg

GTX107945 WB Image

MEKK3 antibody detects MEKK3 protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg whole cell extract of 3xFlag-human MAP3K3-transfected 293T cells
7.5 % SDS-PAGE
MEKK3 antibody (GTX107945) dilution: 1:20000

SDS
Glycerol.pdf
Thimerosal.pdf