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MERS-CoV Envelope protein antibody

Anti-MERS-CoV Envelope protein antibody used in Western Blot (WB). GTX135382
Anti-MERS-CoV Envelope protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135382

Cat. No. GTX135382

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Middle East respiratory syndrome coronavirus
Package
100 μl ($399),
25 μl ($169)
See all MERS-CoV Envelope protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Middle East respiratory syndrome coronavirus

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of MERS-CoV Envelope protein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2909851

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-MERS-CoV Envelope protein antibody used in Western Blot (WB). GTX135382

GTX135382 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with MERS-CoV Envelope protein antibody (GTX135382) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MERS-CoV Envelope protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135382

GTX135382 ICC/IF Image

MERS-CoV Envelope protein antibody detects MERS-CoV Envelope protein protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MERS-CoV Envelope protein stained by MERS-CoV Envelope protein antibody (GTX135382) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169