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MERS-CoV Nucleoprotein antibody

Anti-MERS-CoV Nucleoprotein antibody used in Western Blot (WB). GTX134868
Anti-MERS-CoV Nucleoprotein antibody used in Western Blot (WB). GTX134868
Anti-MERS-CoV Nucleoprotein antibody used in ELISA (ELISA). GTX134868

Cat No. GTX134868

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA

Reactivity

Middle East respiratory syndrome coronavirus
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ELISA Assay dependent
Not tested in other applications.

Positive Control

DDDDK-tag tagged MERS-CoV Nucleoprotein-transfected 293T

Product Note

This antibody detects MERS-CoV Nucleoprotein, but does not cross-react with SARS-CoV or SARS-CoV-2 nucleocapsid proteins based on our internal testing.

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Middle East respiratory syndrome coronavirus

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of MERS-CoV Nucleoprotein (MERS-CoV (strain United Kingdom/H123990006/2012)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887364

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-MERS-CoV Nucleoprotein antibody used in Western Blot (WB). GTX134868

GTX134868 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MERS-CoV Nucleoprotein antibody (GTX134868) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MERS-CoV Nucleoprotein antibody used in Western Blot (WB). GTX134868

GTX134868 WB Image

Non-infected and infected Vero B4 whole cell extracts (20 μg) were separated by gradient gel, and the membrane was blotted with MERS-CoV Nucleoprotein antibody (GTX134868) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MERS-CoV Nucleoprotein antibody used in ELISA (ELISA). GTX134868

GTX134868 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant MERS-CoV Nucleocapsid protein (GTX135653-pro) (88.75-1.39 nM). Coated protein was probed with MERS-CoV Nucleoprotein antibody (GTX134868) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

REFERENCE

There are currently no references for MERS-CoV Nucleoprotein antibody (GTX134868). Be the first to share your publications with this product.

REVIEW

MERS-CoV Nucleoprotein antibody Cat No. GTX134868

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 21-Jan-2020
Score :
Application Tested : WB
Sample Species : MERS-CoV
Sample : Vero B4 cells
Amount used : 20 μg
Blocking : 5% milk in PBST, 20°C, 0.5Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf