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MERS-CoV ORF4a antibody

Anti-MERS-CoV ORF4a antibody used in Western Blot (WB). GTX134869
Anti-MERS-CoV ORF4a antibody used in Western Blot (WB). GTX134869

Cat No. GTX134869

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Middle East respiratory syndrome coronavirus
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
Not tested in other applications.

Positive Control

DDDDK-tag tagged MERS-CoV ORF4a-transfected 293T

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.050 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Middle East respiratory syndrome coronavirus

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of MERS-CoV ORF4a. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-MERS-CoV ORF4a antibody used in Western Blot (WB). GTX134869

GTX134869 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with MERS-CoV ORF4a antibody (GTX134869) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MERS-CoV ORF4a antibody used in Western Blot (WB). GTX134869

GTX134869 WB Image

Non-infected and infected Vero B4 whole cell extracts (20 μg) were separated by gradient gel, and the membrane was blotted with MERS-CoV ORF4a antibody (GTX134869) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

MERS-CoV ORF4a antibody Cat No. GTX134869

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 21-Jan-2020
Score :
Application Tested : WB
Sample Species : MERS-CoV
Sample : Vero B4 cells
Amount used : 20 μg
Blocking : 5% milk in PBST, 20°C, 0.5Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf