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MERS-CoV ORF4a antibody

Anti-MERS-CoV ORF4a antibody used in Western Blot (WB). GTX134869
Anti-MERS-CoV ORF4a antibody used in Western Blot (WB). GTX134869

Cat No. GTX134869

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Middle East respiratory syndrome coronavirus
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
Not tested in other applications.

Positive Control

DDDDK-tag tagged MERS-CoV ORF4a-transfected 293T

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.050 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Middle East respiratory syndrome coronavirus

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of MERS-CoV ORF4a (MERS-CoV (strain United Kingdom/H123990006/2012)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887365

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-MERS-CoV ORF4a antibody used in Western Blot (WB). GTX134869

GTX134869 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with MERS-CoV ORF4a antibody (GTX134869) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MERS-CoV ORF4a antibody used in Western Blot (WB). GTX134869

GTX134869 WB Image

Non-infected and infected Vero B4 whole cell extracts (20 μg) were separated by gradient gel, and the membrane was blotted with MERS-CoV ORF4a antibody (GTX134869) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

MERS-CoV ORF4a antibody Cat No. GTX134869

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 21-Jan-2020
Score :
Application Tested : WB
Sample Species : MERS-CoV
Sample : Vero B4 cells
Amount used : 20 μg
Blocking : 5% milk in PBST, 20°C, 0.5Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf