MERS-CoV ORF4b antibody

Name: MERS-CoV ORF4b antibody
Brand: GeneTex
SKU: GTX134870
Price: 329.00 USD
Rating: 5 (1 reviews)

Anti-MERS-CoV ORF4b antibody used in Western Blot (WB). GTX134870

Cat No. GTX134870

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB
Reactivity	Middle East respiratory syndrome coronavirus
Package	100 μl ($329), 25 μl ($169)

See all MERS-CoV ORF4b products

Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:1000

Not tested in other applications.

Positive Control

293T , DDDDK-tagged MERS-CoV ORF4b-transfected 293T

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Middle East respiratory syndrome coronavirus

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of MERS-CoV ORF4b. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Research Area

DATA IMAGES

GTX134870 WB Image

Non-infected and infected Vero B4 whole cell extracts (20 μg) were separated by gradient gel, and the membrane was blotted with MERS-CoV ORF4b antibody (GTX134870) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX134870 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MERS-CoV ORF4b antibody (GTX134870) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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