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MERS-CoV Spike (S1) antibody

Anti-MERS-CoV Spike (S1) antibody used in Western Blot (WB). GTX134875
Anti-MERS-CoV Spike (S1) antibody used in Western Blot (WB). GTX134875

Cat. No. GTX134875

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Middle East respiratory syndrome coronavirus
Package
100 μl ($399),
25 μl ($169)
See all MERS-CoV Spike products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000
Not tested in other applications.

Positive Control

MERS-CoV (NCoV / Novel coronavirus) Spike Protein-tagged protein-transfected 293T

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.86 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Middle East respiratory syndrome coronavirus

Immunogen

Recombinant protein encompassing a sequence within the center region of MERS-CoV Spike (S1) (MERS-CoV (strain United Kingdom/H123990006/2012)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887370

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Research Area

DATA IMAGES

Anti-MERS-CoV Spike (S1) antibody used in Western Blot (WB). GTX134875

GTX134875 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MERS-CoV S1 protein antibody (GTX134875) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MERS-CoV Spike (S1) antibody used in Western Blot (WB). GTX134875

GTX134875 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MERS-CoV S1 protein antibody (GTX134875) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169