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MERS-CoV Spike overexpression 293T whole cell lysate

MERS-CoV Spike overexpression 293T whole cell lysate. GTX535667

Cat No. GTX535667

Application

WB

Species

Middle East respiratory syndrome coronavirus
Package
200 μg ($299)
PRODUCT

Summary

Whole-cell lysate of 293T cells overexpressing MERS-CoV spike protein (full-length, N-terminal Flag-tagged). The translated amino acid sequence is based on AFS88936.1. Lysate prepared in RIPA buffer with no reducing agents and is unboiled. Please see matched control 293T lysate (GTX535673)

PROPERTIES

Form

Liquid

Buffer

50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 0.5% sodium deoxycholate, 1% Triton X-100, 0.25% SDS, 1 mM PMSF, 5 μg/ml Leupeptin, 2 μg/ml Aprotinin, 1 μg/ml Pepstatin A, 0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

MERS-CoV Spike overexpression 293T whole cell lysate. GTX535667

GTX535667 WB Image

Non-transfected and transfected 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with MERS-CoV Spike (S1) antibody (GTX134875) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Proclin.pdf