GeneTex
United States (US)

MEST antibody, Internal

GTX88397_2338_WB_18122102_593.jpg

Cat No. GTX88397

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.2-0.5μg/ml
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88397-PEP).

Specificity/Sensitivity

This antibody is expected to recognize both reported isoforms (NP_002393.2; NP_803490.1). Reported variants NP_803490.1 and NP_803491.1 represent identical protein.

Predict Reactivity

Human, Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-QELLYRYKQNRSGR, from the internal region of the protein sequence according to NP_002393.2; NP_803490.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mesoderm Specific Transcript,Peg1,Mest

Background

This gene encodes a member of the alpha/beta hydrolase superfamily. It is imprinted, exhibiting preferential expression from the paternal allele in fetal tissues, and isoform-specific imprinting in lymphocytes. The loss of imprinting of this gene has been linked to certain types of cancer and may be due to promotor switching. The encoded protein may play a role in development. Alternatively spliced transcript variants encoding multiple isoforms have been identified for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3 and 4, and the long arm of chromosomes 6 and 15. [provided by RefSeq, Dec 2011]

Database

DATA IMAGES
GTX88397_2338_WB_18122102_593.jpg

GTX88397 WB Image

WB analysis of mouse testis lysate using GTX88397 MEST antibody, Internal.
Dilution : 0.2µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329