GeneTex
United States (US)

METTL3 antibody [N2C2], Internal

GTX105037_WB_19011506_610.jpg

Cat No. GTX105037

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human METTL3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

IME4 antibody, M6A antibody, MGC4336 antibody, MT-A70 antibody, Spo8 antibody, METTL3 antibody, mRNA m(6)A methyltransferase antibody, methyltransferase-like protein 3 antibody, adoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase antibody, N6-adenosine-methyltransferase 70 kDa subunit antibody, methyltransferase like 3 antibody

Cellular Localization

Nucleus speckle

Background

This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine. [provided by RefSeq]

Database

DATA IMAGES
GTX105037_WB_19011506_610.jpg

GTX105037 WB Image

Sample (30 µg of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX105037 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169