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METTL3 antibody

Anti-METTL3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33315
Anti-METTL3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33315
Anti-METTL3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33315
Anti-METTL3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33315
Anti-METTL3 antibody used in Western Blot (WB). GTX33315
Anti-METTL3 antibody used in Immunoprecipitation (IP). GTX33315
Anti-METTL3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33315

Cat No. GTX33315

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:20-1:50
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-156 of human METTL3 (NP_062826.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

methyltransferase like 3 , IME4 , M6A , MT-A70 , Spo8 , hMETTL3

Cellular Localization

Nucleus,Nucleus speckle,Cytoplasm

Background

This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES

Anti-METTL3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33315

GTX33315 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX33315 METTL3 antibody.
Blue : DAPI
Dilution : 1:200

Anti-METTL3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33315

GTX33315 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX33315 METTL3 antibody.
Blue : DAPI
Dilution : 1:100

Anti-METTL3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33315

GTX33315 ICC/IF Image

ICC/IF analysis of C6 cells using GTX33315 METTL3 antibody.
Blue : DAPI
Dilution : 1:100

Anti-METTL3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33315

GTX33315 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX33315 METTL3 antibody.
Blue : DAPI
Dilution : 1:100

Anti-METTL3 antibody used in Western Blot (WB). GTX33315

GTX33315 WB Image

WB analysis of various sample lysates using GTX33315 METTL3 antibody. The signal was developed with ECL plus-Enhanced.
Loading : 25ug per lane
Dilution : 1:1000

Anti-METTL3 antibody used in Immunoprecipitation (IP). GTX33315

GTX33315 IP Image

IP analysis of 293T cells using GTX33315 METTL3 antibody.
IP reaction : 150μg extracts / 3μg antibody antibody
Dilution : 1:1000

Anti-METTL3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33315

GTX33315 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX33315 METTL3 antibody.
Blue : DAPI
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329