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MEX3B antibody

Anti-MEX3B antibody used in Western Blot (WB). GTX32049
Anti-MEX3B antibody used in IHC (Paraffin sections) (IHC-P). GTX32049

Cat. No. GTX32049

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all MEX3B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
IHC-P 20 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

Human Testis Tissue Lysate(GTX15374)

Product Note

This antibody will not recognize the other RKHD3 family of proteins.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

MEX3B antibody was raised against a 16 amino acid synthetic peptide from near the center of human MEX3B.The immunogen is located within amino acids 180 - 230 of MEX3B.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mex-3 RNA binding family member B , MEX-3B , RKHD3 , RNF195

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes an RNA-binding phosphoprotein that is part of the MEX3 (muscle excess 3) family of translational regulators. The encoded protein contains N-terminal nuclear export and nuclear localization signals and is exported from the cytoplasm to the nucleus. The protein binds to RNA via two KH domains and also colocalizes with MEX3A, Dcp1A decapping factor and Argonaute proteins within P (processing) bodies. [provided by RefSeq, Oct 2012]

Database

Research Area

DATA IMAGES

Anti-MEX3B antibody used in Western Blot (WB). GTX32049

GTX32049 WB Image

WB analysis of human testis tissue lysate using GTX32049 MEX3B antibody.
Working concentration : 1 μg/ml

Anti-MEX3B antibody used in IHC (Paraffin sections) (IHC-P). GTX32049

GTX32049 IHC-P Image

IHC-P analysis of mouse skeletal muscle tissue using GTX32049 MEX3B antibody.
Working concentration : 20 μg/ml

REFERENCE

Application Reference

Umehara T et al. Sci Rep 2020; 10 (1) : 15432 Body temperature-dependent microRNA expression analysis in rats: rno-miR-374-5p regulates apoptosis in skeletal muscle cells via Mex3B under hypothermia.
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399