APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 - 2 μg/mL |
| 2.5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
MDA-MB-361 Cell Lysate(GTX23953) , Human Small Intestine Tissue Slide
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Rkhd1 antibody was raised against a 15 amino acid synthetic peptide from near the center of human Rkhd1.The immunogen is located within amino acids 360 - 410 of Rkhd1.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
mex-3 RNA binding family member D , MEX-3D , MEX3 , OK/SW-cl.4 , RKHD1 , RNF193 , TINO
Cellular Localization
Cytoplasm,Nucleus
Background
Rkhd1, also known as TINO or MEX3D is a member of a novel family of four homologous human MEX3 proteins each containing two heterogeneous nuclear ribonucleoprotein K homology (KH) domains and one carboxy-terminal RING finger module. MEX3 proteins, including Rkhd1, are phosphoproteins that bind RNA through their KH domains and shuttle between the nucleus and the cytoplasm via the CRM1 export pathway. These proteins are a novel family of evolutionarily conserved RNA-binding proteins, differentially recruited to P bodies and potentially involved in post-transcriptional regulatory mechanisms. Rkhd1 binds to the AU-rich element in the 3’-untranslated region of Bcl-2 mRNA and is thought to be a negative regulator of Bcl-2 expression. Rkhd3 and Rkhd4, but not Rkhd1, co-localize with both the hDcp1a decapping factor and Argonaute (Ago) proteins in processing bodies (P bodies), recently characterized as centers of mRNA turnover.
Database
Research Area
DATA IMAGES
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GTX85026 IHC-P Image
IHC-P analysis of human small intestine tissue using GTX85026 MEX3D antibody.
Working concentration : 20 μg/ml
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GTX85026 IHC-P Image
IHC-P analysis of human small intestine tissue using GTX85026 MEX3D antibody.
Working concentration : 2.5 μg/ml
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GTX85026 WB Image
WB analysis of MDA-MB-361 cell lysate using GTX85026 MEX3D antibody.
Working concentration : (A) 1 and (B) 2 μg/ml
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REFERENCE
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REVIEW
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