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MFAP5 antibody

Anti-MFAP5 antibody used in Western Blot (WB). GTX48656

Cat. No. GTX48656

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA

Reactivity

 Human, Chimpanzee
Package
50 μg ($399)
See all MFAP5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:250-1:1000
ELISA 1:5000-1:20000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to a region near the carboxy terminal end of human MAGP-2 protein.

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

microfibril associated protein 5 , AAT9 , MAGP-2 , MAGP2 , MFAP-5 , MP25

Cellular Localization

Secreted,Extracellular space,Extracellular matrix

Background

MFAP5 (also known as Microfibril-associated glycoprotein 2, MFAP-2, MP25 and Microfibrillar-associated protein 5 precursor) is a secreted, extracellular matrix protein which is a component of the elastin-associated microfibrils. It forms intermolecular disulfide bonds either with other MFAP5 molecules or with other components of the microfibrils. MFAP5 is upregulated in some ovarian cancers and is associated with poor survival of ovarian cancer patients.

Database

Research Area

DATA IMAGES

Anti-MFAP5 antibody used in Western Blot (WB). GTX48656

GTX48656 WB Image

Western blot using GeneTex's affinity purified anti-MAGP-2 antibody shows detection (arrowhead) of secreted MAGP-2 (lane 2) and MAGP-2 present in a MAGP-2 transfected HEK293 lysate (lane 3). No staining is detected in supernatants from non-transfected cells (lane 1). After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:100 in TBST with 5% BSA overnight at 4º C.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399