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MFAP5 antibody

Anti-MFAP5 antibody used in Western Blot (WB). GTX48656

Cat No. GTX48656

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA

Reactivity

Human, Chimpanzee
Package
50 μg ($289)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:250 - 1:1000
ELISA 1:5000 - 1:20000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminal end of human MFAP5 protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

microfibril associated protein 5 , AAT9 , MAGP-2 , MAGP2 , MFAP-5 , MP25

Background

MFAP5 (also known as Microfibril-associated glycoprotein 2, MFAP-2, MP25 and Microfibrillar-associated protein 5 precursor) is a secreted, extracellular matrix protein which is a component of the elastin-associated microfibrils. It forms intermolecular disulfide bonds either with other MFAP5 molecules or with other components of the microfibrils. MFAP5 is upregulated in some ovarian cancers and is associated with poor survival of ovarian cancer patients.

Database

Research Area

DATA IMAGES

Anti-MFAP5 antibody used in Western Blot (WB). GTX48656

GTX48656 WB Image

Western blot using GeneTex's affinity purified anti-MAGP-2 antibody shows detection (arrowhead) of secreted MAGP-2 (lane 2) and MAGP-2 present in a MAGP-2 transfected HEK293 lysate (lane 3). No staining is detected in supernatants from non-transfected cells (lane 1). After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:100 in TBST with 5% BSA overnight at 4º C.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf