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MFF antibody [HL1311]

Anti-MFF antibody [HL1311] used in IHC (Paraffin sections) (IHC-P). GTX636729
Anti-MFF antibody [HL1311] used in Western Blot (WB). GTX636729
Anti-MFF antibody [HL1311] used in IHC (Paraffin sections) (IHC-P). GTX636729
Anti-MFF antibody [HL1311] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636729

Cat. No. GTX636729

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1311

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MFF. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910060

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mitochondrial fission factor , C2orf33 , EMPF2 , GL004

Background

This is a nuclear gene encoding a protein that functions in mitochondrial and peroxisomal fission. The encoded protein recruits dynamin-1-like protein (DNM1L) to mitochondria. There are multiple pseudogenes for this gene on chromosomes 1, 5, and X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]

Database

Research Area

DATA IMAGES

Anti-MFF antibody [HL1311] used in IHC (Paraffin sections) (IHC-P). GTX636729

GTX636729 IHC-P Image

MFF antibody [HL1311] detects MFF protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
MFF stained by MFF antibody [HL1311] (GTX636729) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MFF antibody [HL1311] used in Western Blot (WB). GTX636729

GTX636729 WB Image

U87-MG whole cell and membrane extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MFF antibody [HL1311] (GTX636729) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MFF antibody [HL1311] used in IHC (Paraffin sections) (IHC-P). GTX636729

GTX636729 IHC-P Image

MFF antibody [HL1311] detects MFF protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
MFF stained by MFF antibody [HL1311] (GTX636729) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MFF antibody [HL1311] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636729

GTX636729 ICC/IF Image

MFF antibody [HL1311] detects MFF protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: MFF stained by MFF antibody [HL1311] (GTX636729) diluted at 1:4500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

There are currently no references for MFF antibody [HL1311] (GTX636729). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for MFF antibody [HL1311] (GTX636729). Be the first to share your experience with this product.
Package List Price ($)
$ 399
$ 169
Custom Conjugations