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MFF antibody [HL1312]

Recombinant antibody Orthogonal
Anti-MFF antibody [HL1312] used in Western Blot (WB). GTX636730
Anti-MFF antibody [HL1312] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636730
Anti-MFF antibody [HL1312] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636730

Cat. No. GTX636730

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1312

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MFF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MFF. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910061

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitochondrial fission factor , C2orf33 , EMPF2 , GL004

Background

This is a nuclear gene encoding a protein that functions in mitochondrial and peroxisomal fission. The encoded protein recruits dynamin-1-like protein (DNM1L) to mitochondria. There are multiple pseudogenes for this gene on chromosomes 1, 5, and X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]

Database

Research Area

DATA IMAGES

Anti-MFF antibody [HL1312] used in Western Blot (WB). GTX636730

GTX636730 WB Image

Orthogonal

U87-MG whole cell and membrane extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MFF antibody [HL1312] (GTX636730) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MFF antibody [HL1312] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636730

GTX636730 ICC/IF Image

MFF antibody [HL1312] detects MFF protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: MFF stained by MFF antibody [HL1312] (GTX636730) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-MFF antibody [HL1312] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636730

GTX636730 ICC/IF Image

MFF antibody [HL1312] detects MFF protein at mitochondria by immunofluorescent analysis.
Sample: U87-MG cells were fixed in ice-cold MeOH for 5 min.
Green: MFF stained by MFF antibody [HL1312] (GTX636730) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations