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MFN2 antibody [N1N2], N-term

Anti-MFN2 antibody [N1N2], N-term used in Western Blot (WB). GTX102055
Anti-MFN2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102055

Cat. No. GTX102055

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all MFN2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

MFN2-transfected 293T

Predict Reactivity

Rat, Zebrafish, Bovine, Dog, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human MFN2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618726

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitofusin 2 , CMT2A , CMT2A2 , CMT2A2A , CMT2A2B , CPRP1 , HMSN6A , HSG , MARF

Background

This gene encodes a mitochondrial membrane protein that participates in mitochondrial fusion and contributes to the maintenance and operation of the mitochondrial network. This protein is involved in the regulation of vascular smooth muscle cell proliferation, and it may play a role in the pathophysiology of obesity. Mutations in this gene cause Charcot-Marie-Tooth disease type 2A2, and hereditary motor and sensory neuropathy VI, which are both disorders of the peripheral nervous system. Defects in this gene have also been associated with early-onset stroke. Two transcript variants encoding the same protein have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MFN2 antibody [N1N2], N-term used in Western Blot (WB). GTX102055

GTX102055 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MFN2 antibody [N1N2], N-term (GTX102055) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MFN2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102055

GTX102055 IHC-P Image

MFN2 antibody [N1N2], N-term detects MFN2 protein at cytosol on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
MFN2 antibody [N1N2], N-term (GTX102055) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Sakiyama H et al. Mol Cell Biochem 2021; 476 (10) : 3577-3590 A lack of ChREBP inhibits mitochondrial cristae formation in brown adipose tissue.
Lam HYP et al. Parasitol Int 2021; 80 : 102231 Mitochondrial dynamics in Angiostrongylus cantonensis-infected mouse brain.
Application : WB
Reactivity : Mouse
Liu YH et al. Phytomedicine 2018; 46 : 184-192 Aqueous extracts of Paeonia suffruticosa modulates mitochondrial proteostasis by reactive oxygen species-induced endoplasmic reticulum stress in pancreatic cancer cells.
Application : WB
Reactivity : Human
Li CJ et al. Oxid Med Cell Longev 2017; 2017 : 8510805 Enhancement of Mitochondrial Transfer by Antioxidants in Human Mesenchymal Stem Cells.
Application : WB
Reactivity : Human
Wu LH et al. J Cell Physiol 2017; (Epub) Laminar Shear Stress Promotes Mitochondrial Homeostasis in Endothelial Cells.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169