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MGAT3 antibody [N3C3]

Anti-MGAT3 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112153
Anti-MGAT3 antibody [N3C3] used in Western Blot (WB). GTX112153
Anti-MGAT3 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112153
Anti-MGAT3 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112153

Cat. No. GTX112153

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all MGAT3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

HeLa , HepG2

Predict Reactivity

Rat, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.96 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MGAT3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950913

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase , GNT-III , GNT3

Cellular Localization

Golgi apparatus membrane; Single-pass type II membrane protein

Background

There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. The enzyme encoded by this gene transfers a GlcNAc residue to the beta-linked mannose of the trimannosyl core of N-linked oligosaccharides and produces a bisecting GlcNAc. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MGAT3 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112153

GTX112153 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using GnT-III(GTX112153) antibody at 1:200 dilution.

Anti-MGAT3 antibody [N3C3] used in Western Blot (WB). GTX112153

GTX112153 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2

7.5% SDS PAGE
GTX112153 diluted at 1:1000

Anti-MGAT3 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112153

GTX112153 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using MGAT3(GTX112153) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MGAT3 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112153

GTX112153 IHC-P Image

MGAT3 antibody [N3C3] detects MGAT3 protein at cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
MGAT3 antibody [N3C3] (GTX112153) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Sammartino G et al. Biomed Res Int 2016; 2016 : 5713168 Tissue Engineering and Dental Implantology: Biomaterials, New Technologies, and Stem Cells.
Application : WB
Reactivity : Human
Chen YJ et al. Biomed Res Int 2015; 2015 : 924397 Hexosamine-Induced TGF-β Signaling and Osteogenic Differentiation of Dental Pulp Stem Cells Are Dependent on N-Acetylglucosaminyltransferase V.
Application : WB
Reactivity : Human
Zhang M et al. Hum Reprod 2015; 30 (9) : 2064-75 Altered β1,6-GlcNAc and bisecting GlcNAc-branched N-glycan on integrin β1 are associated with early spontaneous miscarriage in humans.
Application : ICC/IF
Reactivity : Human
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169