APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa
Predict Reactivity
Mouse, Rat, Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human MGAT4A. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10617950
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A , GNT-IV , GNT-IVA , GnT-4a
Cellular Localization
Alpha-1 , 3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A: Golgi apparatus membrane; Single-pass type II membrane protein , 3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A soluble form: Secreted
Background
This gene encodes a key glycosyltransferase that regulates the formation of tri- and multiantennary branching structures in the Golgi apparatus. The encoded protein, in addition to the related isoenzyme B, catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc in a beta-1,4 linkage to the Man-alpha-1,3-Man-beta-1,4-GlcNAc arm of R-Man-alpha-1,6(GlcNAc-beta-1,2-Man-alpha-1,3)Man-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1-Asn. The encoded protein may play a role in regulating the availability of serum glycoproteins, oncogenesis, and differentiation. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX119640 ICC/IF Image
Immunofluorescence analysis of methanol-fixed HeLa, using MGAT4A(GTX119640) antibody at 1:200 dilution.
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GTX119640 IHC-P Image
Immunohistochemical analysis of paraffin-embedded H358 xenograft, using MGAT4A(GTX119640) antibody at 1:250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX119640 WB Image
Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE GTX119640 diluted at 1:1000
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REFERENCE
There are currently no references for MGAT4A antibody [C1C3] (GTX119640). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MGAT4A antibody [C1C3] (GTX119640). Be the first to share your experience with this product.