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MGAT4A antibody [C1C3]

Anti-MGAT4A antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX119640
Anti-MGAT4A antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119640
Anti-MGAT4A antibody [C1C3] used in Western Blot (WB). GTX119640

Cat No. GTX119640

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human MGAT4A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617950

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A , GNT-IV , GNT-IVA , GnT-4a

Cellular Localization

Alpha-1 , 3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A: Golgi apparatus membrane; Single-pass type II membrane protein , 3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A soluble form: Secreted

Background

This gene encodes a key glycosyltransferase that regulates the formation of tri- and multiantennary branching structures in the Golgi apparatus. The encoded protein, in addition to the related isoenzyme B, catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc in a beta-1,4 linkage to the Man-alpha-1,3-Man-beta-1,4-GlcNAc arm of R-Man-alpha-1,6(GlcNAc-beta-1,2-Man-alpha-1,3)Man-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1-Asn. The encoded protein may play a role in regulating the availability of serum glycoproteins, oncogenesis, and differentiation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MGAT4A antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX119640

GTX119640 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H358 xenograft, using MGAT4A(GTX119640) antibody at 1:250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MGAT4A antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119640

GTX119640 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using MGAT4A(GTX119640) antibody at 1:200 dilution.

Anti-MGAT4A antibody [C1C3] used in Western Blot (WB). GTX119640

GTX119640 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
GTX119640 diluted at 1:1000

REFERENCE

There are currently no references for MGAT4A antibody [C1C3] (GTX119640). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for MGAT4A antibody [C1C3] (GTX119640). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf