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MGEA5 antibody

Anti-MGEA5 antibody used in IHC (Paraffin sections) (IHC-P). GTX01028
Anti-MGEA5 antibody used in Western Blot (WB). GTX01028
Anti-MGEA5 antibody used in IHC (Paraffin sections) (IHC-P). GTX01028
Anti-MGEA5 antibody used in Flow cytometry (FACS). GTX01028

Cat No. GTX01028

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, FACS

Reactivity

Human
Package
200 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:2000
IHC-P 1:25
FACS 1:25
Not tested in other applications.

Calculated MW

103 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% (W/V) sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This MGEA5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 236-269 amino acids from the human region of human MGEA5.

Purification

Purified by protein A column, followed by peptide affinity purification.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

O-GlcNAcase,MEA5,MGEA5,NCOAT,OGA,meningioma expressed antigen 5 (hyaluronidase)

Cellular Localization

Nucleus,Cytoplasm

Background

The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM, Mar 2008]

Database

Research Area

DATA IMAGES

Anti-MGEA5 antibody used in IHC (Paraffin sections) (IHC-P). GTX01028

GTX01028 IHC-P Image

IHC-P analysis of human testis tissue sections using GTX01028 MGEA5 antibody.
Antigen retrieval : Heat mediation with a citrate buffer (pH6)
Dilution : 1:25

Anti-MGEA5 antibody used in Western Blot (WB). GTX01028

GTX01028 WB Image

WB analysis of various sample lysates using GTX01028 MGEA5 antibody.
Lane 1 : U-87 MG whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : BxPC-3 whole cell lysate
Dilution : 1:2000
Loading : 20 μg per lane

Anti-MGEA5 antibody used in IHC (Paraffin sections) (IHC-P). GTX01028

GTX01028 IHC-P Image

IHC-P analysis of human pancreas tissue sections using GTX01028 MGEA5 antibody.
Antigen retrieval : Heat mediation with a citrate buffer (pH6)
Dilution : 1:25

Anti-MGEA5 antibody used in Flow cytometry (FACS). GTX01028

GTX01028 FACS Image

FACS analysis of HeLa cells using GTX01028 MGEA5 antibody.
Green : Primary antibdoy
Blue : Isotype control
Dilution : 1μg/1x10⁶ cells
Fixation : 2% paraformaldehyde (10 min)
Permeabilization : 90% methanol for 10 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 339