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MGEA5 antibody

Anti-MGEA5 antibody used in IHC (Paraffin sections) (IHC-P). GTX115467
Anti-MGEA5 antibody used in IHC (Paraffin sections) (IHC-P). GTX115467

Cat. No. GTX115467

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 IHC-P

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all MGEA5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

103 kDa. ( Note )

Predict Reactivity

Human, Zebrafish, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MGEA5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885368

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

O-GlcNAcase , MEA5 , MGEA5 , NCOAT

Cellular Localization

Isoform 3: Nucleus , Isoform 1: Cytoplasm

Background

The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-MGEA5 antibody used in IHC (Paraffin sections) (IHC-P). GTX115467

GTX115467 IHC-P Image

MGEA5 antibody detects MGEA5 protein at cytosol on mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
MGEA5 antibody (GTX115467) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MGEA5 antibody used in IHC (Paraffin sections) (IHC-P). GTX115467

GTX115467 IHC-P Image

MGEA5 antibody detects MGEA5 protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
MGEA5 antibody (GTX115467) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169