GeneTex
United States (US)

MGEA5 antibody

GTX115467_41248_IHC_R_18121410_251.jpg
GTX115467_41248_IHC_M_18121410_650.jpg

Cat No. GTX115467

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application IHC-P
Reactivity Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

103 kDa. ( Note )

Predict Reactivity

Human, Zebrafish, Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MGEA5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

O-Glcnacase,Mea5,Mgea5,Ncoat,Oga

Cellular Localization

Isoform 3: Nucleus , Isoform 1: Cytoplasm

Background

The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX115467_41248_IHC_R_18121410_251.jpg

GTX115467 IHC-P Image

MGEA5 antibody detects MGEA5 protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
MGEA5 antibody (GTX115467) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115467_41248_IHC_M_18121410_650.jpg

GTX115467 IHC-P Image

MGEA5 antibody detects MGEA5 protein at cytosol on mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
MGEA5 antibody (GTX115467) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169