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MGMT antibody [MT 23.2]

Orthogonal
Anti-MGMT antibody [MT 23.2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX27045
Anti-MGMT antibody [MT 23.2] used in Western Blot (WB). GTX27045
Anti-MGMT antibody [MT 23.2] used in IHC (Paraffin sections) (IHC-P). GTX27045
Anti-MGMT antibody [MT 23.2] used in Western Blot (WB). GTX27045
Anti-MGMT antibody [MT 23.2] used in Flow cytometry (FACS). GTX27045
Anti-MGMT antibody [MT 23.2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX27045
Anti-MGMT antibody [MT 23.2] used in Flow cytometry (FACS). GTX27045
Anti-MGMT antibody [MT 23.2] used in Western Blot (WB). GTX27045

Cat. No. GTX27045

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MT 23.2

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, FACS, MS

Reactivity

 Human
Package
100 μl ($409)
See all MGMT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:500
IHC-P 1:500 - 1:2000
FACS 1 - 2 μg/mL
MS Assay dependent
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Product Note

We do not recommend use of this product for Mouse samples.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human MGMT (O6-methylguanine-DNA methyltransferase) purified from E. coli [UniProt# P16455]

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_372329

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

O-6-methylguanine-DNA methyltransferase

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-MGMT antibody [MT 23.2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX27045

GTX27045 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX27045 MGMT antibody [MT 23.2].
Green : primary antibody
Blue : Hoechst 33258
Dilution : 1:50

Anti-MGMT antibody [MT 23.2] used in Western Blot (WB). GTX27045

GTX27045 WB Image

WB analysis of MCF-7 cell lysate using GTX27045 MGMT antibody [MT 23.2].

Anti-MGMT antibody [MT 23.2] used in IHC (Paraffin sections) (IHC-P). GTX27045

GTX27045 IHC-P Image

IHC-P analysis of human kidney tissue using GTX27045 MGMT antibody [MT 23.2].
Dilution : 1:2000
Antigen retrieval : Heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0)

Anti-MGMT antibody [MT 23.2] used in Western Blot (WB). GTX27045

GTX27045 WB Image

WB analysis of MCF-7 cell lysate using GTX27045 MGMT antibody [MT 23.2].
Dilution : 1:1000

Anti-MGMT antibody [MT 23.2] used in Flow cytometry (FACS). GTX27045

GTX27045 FACS Image

FACS (Intracellular staining) analysis of Raji cells using GTX27045 MGMT antibody [MT 23.2].
Blue : Primary antibody
Orange : isotype control
Dilution : 2 μg/mL
Fixation : 4% PFA
Permibilization : 0.1% saponin

Anti-MGMT antibody [MT 23.2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX27045

GTX27045 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX27045 MGMT antibody [MT 23.2].
Green : primary antibody
Red : Actin
Blue : DAPI
Dilution : 1:200
Fixation : 10% formalin
Permibilization : 0.5% Triton-X100

Anti-MGMT antibody [MT 23.2] used in Flow cytometry (FACS). GTX27045

GTX27045 FACS Image

FACS (Intracellular staining) analysis of Jurkat cells using GTX27045 MGMT antibody [MT 23.2].
Blue : Primary antibody
Orange : isotype control
Dilution : 1 μg/mL
Fixation : 4% PFA
Permibilization : 0.1% saponin

Anti-MGMT antibody [MT 23.2] used in Western Blot (WB). GTX27045

GTX27045 WB Image

Orthogonal

The data was published in the journal Oncotarget in 2016. PMID: 27057640

REFERENCE

Application Reference

Khalil S et al. Oncotarget 2016; 7 (19) : 28195-206 miRNA array screening reveals cooperative MGMT-regulation between miR-181d-5p and miR-409-3p in glioblastoma.
Application : WB
Reactivity : Human
Mojas N et al. Genes Dev 2007; 21 (24) : 3342-55 Mismatch repair-dependent processing of methylation damage gives rise to persistent single-stranded gaps in newly replicated DNA.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409