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United States (US)

MGMT antibody [MT3.1]

Cat. No. GTX23144

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MT3.1

Isotype

 IgG1

Application

 IHC-P

Reactivity

 Human
Package
500 μl ($549)
See all MGMT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:20-1:40

Note :

IHC-P
Pretreatment of deparaffinized tissue with heat-induced epitope retrieval is recommended.
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

Colon carcinoma

PROPERTIES

Form

Liquid

Buffer

Tris-HCI buffer, 0.2% BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human MGMT protein

Purification

Purified IgG

Conjugation

Unconjugated

RRID

AB_372330

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

O-6-methylguanine-DNA methyltransferase

Cellular Localization

Nucleus

Background

Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by this gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma. [provided by RefSeq, Sep 2015]

Database

Research Area

REFERENCE

Application Reference

Neto JC et al. Pathol Res Pract 2011; 207 (8) : 492-7 Evaluation of O6-methylguanine-DNA methyltransferase by immunohistochemistry: best clinical and research practices.
Application : IHC-P
Reactivity : Human
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Package List Price ($)
$ 549