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MGMT antibody

Orthogonal
Anti-MGMT antibody used in Immunoprecipitation (IP). GTX110551
Anti-MGMT antibody used in Western Blot (WB). GTX110551

Cat. No. GTX110551

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MGMT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MGMT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950916

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

O-6-methylguanine-DNA methyltransferase

Cellular Localization

Nucleus

Background

Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by this gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-MGMT antibody used in Immunoprecipitation (IP). GTX110551

GTX110551 IP Image

Immunoprecipitation of MGMT protein from Jurkat whole cell extracts using 5 μg of MGMT antibody (GTX110551).
Western blot analysis was performed using MGMT antibody (GTX110551) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-MGMT antibody used in Western Blot (WB). GTX110551

GTX110551 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MGMT antibody (GTX110551) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169