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MGST1 antibody

Anti-MGST1 antibody used in Western Blot (WB). GTX114551
Anti-MGST1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114551
Anti-MGST1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114551

Cat No. GTX114551

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Positive Control

HeLa , HepG2 , Mouse liver , Rat liver

Predict Reactivity

Bovine, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.41 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MGST1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11176921

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

microsomal glutathione S-transferase 1 , GST12 , MGST , MGST-I

Cellular Localization

Microsome , Mitochondrion outer membrane; Peripheral membrane protein , Endoplasmic reticulum

Background

The MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) family consists of six human proteins, two of which are involved in the production of leukotrienes and prostaglandin E, important mediators of inflammation. Other family members, demonstrating glutathione S-transferase and peroxidase activities, are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. This gene encodes a protein that catalyzes the conjugation of glutathione to electrophiles and the reduction of lipid hydroperoxides. This protein is localized to the endoplasmic reticulum and outer mitochondrial membrane where it is thought to protect these membranes from oxidative stress. Four transcript variants of this gene encode one protein isoform. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MGST1 antibody used in Western Blot (WB). GTX114551

GTX114551 WB Image

MGST1 antibody detects MGST1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with MGST1 antibody (GTX114551) diluted at a dilution of 1:1000.

Anti-MGST1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114551

GTX114551 IHC-P Image

MGST1 antibody [N1C3] detects MGST1 protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
MGST1 antibody [N1C3] (GTX114551) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MGST1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114551

GTX114551 IHC-P Image

MGST1 antibody [N1C3] detects MGST1 protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
MGST1 antibody [N1C3] (GTX114551) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf